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Total glutathione lysis reagent and GSSG lysis reagent, which contains alkylating agent N-ethylmaleimide (25 mM) were prepared following manufactures protocol and added to respective wells along with blank for background

Alpha Lipoic Acid is a great promoter of intracellular glutathione

(Micha Karpiski), J.M., A.S., Z.S., B.Z., W.L., J.C.-P

Similarly, under oxidative stress, EGCG not only failed to provide protection in hydrogen peroxide-induced model but also worsened mitochondrial membrane potential loss, caspase-3 activation, and DNA fragmentation, suggesting potential pro-apoptotic and mitochondria-damaging effects under such conditions (Sahadevan et al., 2023)

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